Quantification of dissociation constant in living cells by fluorescence cross-correlation spectroscopy
نویسندگان
چکیده
منابع مشابه
Determination of dissociation constant of the NFκB p50/p65 heterodimer using fluorescence cross-correlation spectroscopy in the living cell.
Two-laser-beam fluorescence cross-correlation spectroscopy (FCCS) is promising technique that provides quantitative information about the interactions of biomolecules. The p50/p65 heterodimer is the most abundant and well understood of the NFκB dimers in most cells. However, the quantitative value of affinity, namely the K(d), for the heterodimer in living cells is not known yet. To quantify th...
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ژورنال
عنوان ژورنال: Folia Pharmacologica Japonica
سال: 2016
ISSN: 0015-5691,1347-8397
DOI: 10.1254/fpj.147.74